1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

gen. nov. sp. nov., sp. nov. and gen. nov. sp. nov., isolated from human faeces No Access

Abstract

Three Gram-positive, obligately anaerobic bacterial strains, namely CSJ-1, CSJ-3, and CSJ-4, were isolated from faeces of healthy persons. They were characterized through a combination of whole-genome sequencing, phenotypic traits, and metabolomic analysis. The genome sizes of CSJ-1, CSJ-4, and CSJ-3 were 3.3, 3.8, and 6.1 Mbp, with DNA G+C contents of 47.2, 48.3, and 48.8 mol%, respectively. Strain CSJ-3 was identified as representing a novel species, (type strain CSJ-3=CGMCC 1.18033=JCM 36512) of the genus . The 16S rRNA gene sequence similarity and whole genome average nucleotide identity (gANI) of CSJ-4 to its closest related species, ASD 4241, were 98.3 and 91.4 %, respectively. Comparative analysis of 16S rRNA gene sequences showed 91.6 % similarity between CSJ-1 and its closest phylogenetic neighbour, DSM 106146, and 93.3 % similarity between CSJ-4 and its closest relative strain, SNUG30386. Based on the polyphasic taxonomic results, we proposed two novel genera and three novel species. Strain CSJ-1 was identified as representing a novel species of novel genus, gen. nov. sp. nov. (type strain CSJ-1=CGMCC 1.18046=JCM 36511) of the family , and strain CSJ-4 was identified as representing a novel species of novel genus gen. nov. sp. nov. (type strain CSJ-4=CGMCC 1.18026= JCM 36513) of the family .

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  • Accepted:
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Keyword(s): Anaerolentibacter hominis , Clostridiaceae , Diplocloster hominis , gut microbiome , Lachnospiraceae and Pilosibacter fragilis
Funding
This study was supported by the:
  • the State Key Laboratory of Microbial Technology Open Projects Fund (Award M2021-03)
    • Principle Award Recipient: Shuang-jiangLiu
  • the National Key Research and Development Program of China (Award 2022YFA1304103)
    • Principle Award Recipient: Shuang-jiangLiu
© 2024 The Authors
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2024-04-30
2024-05-17
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Anaerolentibacter hominis gen. nov. sp. nov., Diplocloster hominis sp. nov. and Pilosibacter fragilis gen. nov. sp. nov., isolated from human faeces
Int J Syst Evol Microbiol 74, 006359 (2024); https://doi.org/10.1099/ijsem.0.006359
/content/journal/ijsem/10.1099/ijsem.0.006359
/content/journal/ijsem/10.1099/ijsem.0.006359
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