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Abstract
The taxonomic position of three actinobacterial strains, BCCO 10_0061T, BCCO 10_0798T, and BCCO 10_0856T, recovered from bare soil in the Sokolov Coal Basin, Czech Republic, was established using a polyphasic approach. The multilocus sequence analysis based on 100 single-copy genes positioned BCCO 10_0061T in the same cluster as Lentzea waywayandensis, strain BCCO 10_0798T in the same cluster as Lentzea flaviverrucosa, Lentzea californiensis, Lentzea violacea, and Lentzea albidocapillata, and strain BCCO 10_0856T clustered together with Lentzea kentuckyensis and Lentzea alba. Morphological and chemotaxonomic characteristics of these strains support their assignment to the genus Lentzea. In all three strains, MK-9(H4) accounted for more than 80 % of the isoprenoid quinone. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The whole-cell sugars were rhamnose, ribose, mannose, glucose, and galactose. The major fatty acids (>10 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, and C16 : 0. The polar lipids were diphosphatidylglycerol, methyl-phosphatidylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol. The genomic DNA G+C content of strains (mol%) was 68.8 for BCCO 10_0061T, 69.2 for BCCO 10_0798T, and 68.5 for BCCO 10_0856T. The combination of digital DNA–DNA hybridization results, average nucleotide identity values and phenotypic characteristics of BCCO 10_0061T, BCCO 10_0798T, and BCCO 10_0856T distinguishes them from their closely related strains. Bioinformatic analysis of the genome sequences of the strains revealed several biosynthetic gene clusters (BGCs) with identities >50 % to already known clusters, including BGCs for geosmin, coelichelin, ε-poly-l-lysine, and erythromycin-like BGCs. Most of the identified BGCs showed low similarity to known BGCs (<50 %) suggesting their genetic potential for the biosynthesis of novel secondary metabolites. Based on the above results, each strain represents a novel species of the genus Lentzea, for which we propose the name Lentzea sokolovensis sp. nov. for BCCO 10_0061T (=DSM 116175T), Lentzea kristufekii sp. nov. for BCCO 10_0798T (=DSM 116176T), and Lentzea miocenica sp. nov. for BCCO 10_0856T (=DSM 116177T).
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Funding
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Leibniz Association through the Collaborative Excellence
(Award K445/2022)
- Principle Award Recipient: YvonneMast
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Deutsches Zentrum für Infektionsforschung
(Award TTU 09.819)
- Principle Award Recipient: YvonneMast
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Ministerstvo školství, mládeže a tělovýchovy České republiky
(Award OP VVV CZ.02.2.69/0.0/0.0/18_054/0014649, ABERA - Anchoring the Biological Centre AV ČR, v. v. i. in the European Research Area)
- Principle Award Recipient: ApplicableNot
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Ministerstvo školství, mládeže a tělovýchovy České republiky
(Award OP VVV CZ.02.1.01/0.0/0.0/18_046/0016045 and Czech-BioImaging LM2023050)
- Principle Award Recipient: ApplicableNot
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Akademie věd České republiky
(Award Strategie AV21)
- Principle Award Recipient: ChroňákováAlica
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Ministerstvo Zdravotnictví České Republiky
(Award No. 17-30091A)
- Principle Award Recipient: ChroňákováAlica