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Volume 74, Issue 5

sp. nov., a halotolerant actinobacterium with antimicrobial activity No Access

Abstract

A novel Gram-staining-positive actinobacterium with antimicrobial activity, designated CFH 90308, was isolated from the sediment of a salt lake in Yuncheng, Shanxi, south-western China. The isolate exhibited the highest 16S rRNA gene sequence similarities to G72, NBRC 15708 and S3-E (98.5, 98.4 and 98.2 %, respectively), and formed a separate clade with S3-E in phylogenetic trees. The strain grew at 15–40 ºC, pH 6.0–8.0 and could tolerate NaCl up to a concentration of 15 % (w/v). The whole genome of strain CFH 90308 consisted of 4.33 Mbp and the DNA G+C content was 69.6 mol%. The acyl type of the peptidoglycan was glycolyl and the whole-cell sugars were galactose and mannose. The cell-wall peptidoglycan mainly contained alanine, glycine and lysine. The menaquinones of strain CFH 90308 were MK-12, MK-13 and MK-11. Strain CFH 90308 contained anteiso-C, anteiso-C, iso-C and iso-C as the predominant fatty acids. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between CFH 90308 and the other species of the genus were found to be low (ANIb <81.3 %, dDDH <25.6 %). The secondary metabolite produced by strain CFH 90308 showed antibacterial activities against , , and methicillin-resistant . Based on genotypic, phenotypic and chemotaxonomic results, the isolate is considered to represent a novel species of the genus , for which the name sp. nov is proposed. The type strain is CFH 90308 (=DSM 105964=KCTC 49052).

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Keyword(s): antimicrobial activity , Microbacterium salsuginis sp. nov. , phylogenetic analysis and polyphasic taxonomy
Funding
This study was supported by the:
  • Key Technologies R&D Program of Henan Province (Award 232102311146)
    • Principle Award Recipient: CaixiaCui
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: HongMing
© 2024 The Authors
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Microbacterium salsuginis sp. nov., a halotolerant actinobacterium with antimicrobial activity
Int J Syst Evol Microbiol 74, 006362 (2024); https://doi.org/10.1099/ijsem.0.006362
/content/journal/ijsem/10.1099/ijsem.0.006362
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