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Volume 74, Issue 5

sp. nov., isolated from the breast milk of a healthy woman No Access

Abstract

Human breast milk contains lactic acid bacteria (LAB), which have an important influence on the composition of the intestinal microbia of infants. In this study, one strain of an α-hemolytic species of the genus , IMAU99199, isolated from the breast milk of a healthy nursing mother in Hohhot city PR China, was studied to characterise its taxonomic status using phenotypic and molecular taxonomic methods. The results indicated that it represented a member of the mitis–suis clade, pneumoniae subclade of the genus . It is a Gram-stain-positive, catalase-negative and oxidase-negative bacterium, and the cells are globular, paired or arranged in short chains. The results of a phylogenetic analysis of its 16S rRNA gene and two housekeeping genes ( and ) placed it in the genus . A phylogenetic tree based on 135 single-copy genes sequences indicated that IMAU99199 formed a closely related branch well separated from ‘’ IMAU99125, ‘’ Marseille Q6994, NCTC 12261, ‘’ DM3B3, TP1632, ATCC BAA-960 and NCTC 7465. IMAU99199 and ‘’ IMAU99125 had the highest average nucleotide identity (93.7 %) and digital DNA–DNA hybridisation (55.3 %) values, which were below the accepted thresholds for novel species. The DNA G+C content of the draft genome of IMAU99199 was 39.8 %. The main cellular fatty acids components of IMAU99199 were C and Cω7. It grew at a temperature range of 25–45 °C (the optimum growth temperature was 37 °C) and a pH range of 5.0–8.0 (the optimum growth pH was 7.0). These data indicate that strain IMAU99199 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is IMAU99199 (=GDMCC 1.1874=KCTC 21155).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): breast milk , novel species , Streptococcus and Streptococcus hohhotensis sp. nov.
Funding
This study was supported by the:
  • Joint Funds of the National Natural Science Foundation of China (Award U23A20260)
    • Principle Award Recipient: LiuWenjun
  • National Natural Science Foundation of China (Award 32272289)
    • Principle Award Recipient: LiuWenjun
  • Youth Science and Technology Talent Project of Higher Education Institutions in Inner Mongolia Autonomous Region (Award NYT22042)
    • Principle Award Recipient: LiuWenjun
© 2024 The Authors
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Streptococcus hohhotensis sp. nov., isolated from the breast milk of a healthy woman
Int J Syst Evol Microbiol 74, 006354 (2024); https://doi.org/10.1099/ijsem.0.006354
/content/journal/ijsem/10.1099/ijsem.0.006354
/content/journal/ijsem/10.1099/ijsem.0.006354
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