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Volume 74, Issue 4

sp. nov., isolated from sediment in a freshwater pond No Access

Abstract

A Gram-stain-negative, aerobic, oxidase-positive, weakly catalase-positive, motile by means of a single polar flagellum, rod-shaped bacterium designated as strain S2-9 was isolated from sediment sampled in Wiyang pond, Republic of Korea. Growth of this strain was observed at 10–40 °C (optimum, 35 °C) and pH 5.5–9.5 (optimum, pH 7.0–8.0) and in the presence of 0–0.5 % NaCl in Reasoner's 2A broth. The major fatty acids (>10 %) of strain S2-9 were C and summed feature 3 (comprising a mixture of C 7 and/or C 6). Ubiquinone-8 was detected as the respiratory quinone. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Strain S2-9 showed the highest 16S rRNA gene sequence similarity to CHU3 (98.7 %), followed by ‘’ CR182 (98.4 %), all type strains of species (98.1–98.3 %), NBRC 102408 (97.9 %), KIN192 (97.8 %), HWN-4 (97.4 %), and 2C20 (97.4 %). Phylogenetic trees based on the 16S rRNA gene and whole-genome sequences showed that strain S2-9 formed a tight phylogenetic lineage with species (CHU3, CR182, and 2C20). Average nucleotide identity and digital DNA–DNA hybridization values between strain S2-9 and strains were 76.6–79.3% and 19.5–21.5 %, respectively. The genomic DNA G+C content of strain S2-9 was 68.3 mol%. Notably, genes responsible for both sulphur oxidation and reduction and denitrification were found in the genome of strain S2-9, suggesting that strain S2-9 is involved in the nitrogen and sulphur cycles in pond ecosystems. Based on the polyphasic taxonomic results, strain S2-9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2-9 (= KACC 22267= JCM 34541).

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Keyword(s): new taxa , Paucibacter sediminis , pond sediment and taxonomy
© 2024 The Authors
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2024-04-24
2024-05-04
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Paucibacter sediminis sp. nov., isolated from sediment in a freshwater pond
Int J Syst Evol Microbiol 74, 006312 (2024); https://doi.org/10.1099/ijsem.0.006312
/content/journal/ijsem/10.1099/ijsem.0.006312
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